Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TINAG / TIN-AG Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101225-100
Catalog Number: LS-G101225
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TINAG (NM_014464), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TIN-AG | TIN2 | TIN1 | TINAG
- Origin Species
- Human
- NCBI GenBank
- NM_014464
- SwissProt
- Q9UJW2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWTGYKILIF SYLTTEIWME KRYLSQREVD LEAYFTRNHT VLQGTRFKRA IFQGQYCRNF61GCCEDRDDGC VTEFYAANAL CYCDKFCDRE NSDCCPDYKS FCREEKEWPP HTQPWYPEGC121FKDGQHYEEG SVIKENCNSC TCSGQQWKCS QHVCLVRSEL IEQVNKGDYG WTAQNYSQFW181GMTLEDGFKF RLGTLPPSLM LLSMNEMTAS LPATTDLPEF FVASYKWPGW THGPLDQKNC241AASWAFSTAS VAADRIAIQS KGRYTANLSP QNLISCCAKN RHGCNSGSID RAWWYLRKRG301LVSHACYPLF KDQNATNNGC AMASRSDGRG KRHATKPCPN NVEKSNRIYQ CSPPYRVSSN361ETEIMKEIMQ NGPVQAIMQV HEDFFHYKTG IYRHVTSTNK ESEKYRKLQT HAVKLTGWGT421LRGAQGQKEK FWIAANSWGK SWGENGYFRI LRGVNESDIE KLIIAAWGQL TSSDEPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
476 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.