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TIGD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TIGD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106627-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TIGD4 (NM_145720), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TIGD4
Origin Species
Human
NCBI GenBank
AA758641
SwissProt
Q8IY51

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEASVDAST LPVTVKKKKS LSIEEKIDII NAVESGKKKA EIAAEYGIKK NSLSSIMKNK
61DKVLEAFESL RFDPKRKRLR TAFYTDLEEA LMRWYRIAQC LNVPVNGPML RLKANDFAQK
121LGHNDFKCSN GWLDRFKSRY GLVFRAQPVE ATGVPVDPST VWYQNVLPYY LNDYHPKNVF
181NIKETGLLYR MLPTNTFAFK GETCSVGKLC KDRITLVVGT NMDGSEKLPL LVIGKKRTPH
241CFKGLKSLPV CYEANRMAWM TSDVFEQWMR KLDEEFQAQQ RRVVIFVESF PAHPEVKNLK
301SIELAFFPSC LSSKCIAMKQ GVIKSLKIKY RHCLIKKFLS SVEGSKEFTF SLLDAVDTLH
361LCWRAVTPET IVKSYEEAGF KSQKGESDIT NAEKDTGLDL VADALGAGVE FPEGLSIEEY
421AALDDDLETC EAAPNGDSVC TKESKSDETG FYTSDEEDDD GSPGTELPLP SKSEAITALD
481TLKKFLRSQD MNDGLQNSLA DLENFINSLS PK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

512 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.