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TICRR Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TICRR Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TICRR antibodies →
$725.00each
Catalog Number: LS-G106405-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C15orf42(TICRR) (NM_152259), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
210.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MACCHKVMLL LDTAGGAARH SRVRRAALRL LTYLSCRFGL ARVHWAFKFF DSQGARSRPS
61RVSDFRELGS RSWEDFEEEL EARLEDRAHL PGPAPRATHT HGALMETLLD YQWDRPEITS
121PTKPILRSSG RRLLDVESEA KEAEAALGGL VNAVFLLAPC PHSQRELLQF VSGCEAQAQR
181LPPTPKQVME KLLPKRVREV MVARKITFYW VDTTEWSKLW ESPDHLGYWT VCELLHHGGG
241TVLPSESFSW DFAQAGEMLL RSGIKLSSEP HLSPWISMLP TDATLNRLLY NSPEYEASFP
301RMEGMLFLPV EAGKEIQETW TVTLEPLAMH QRHFQKPVRI FLKGSVAQWS LPTSSTLGTD
361SWMLGSPEES TATQRLLFQQ LVSRLTAEEL HLVADVDPGE GWPPITGVIS PLSASAMILT
421VCRTKEAEFQ RHVLQTAVAD SPRDTASLFS DVVDSILNQT HDSLADTASA ASPVPEWAQQ
481ELGHTTPWSP AVVEKWFPFC NISGASSDLM ESFGLLQAAS ANKEESSKTE GELIHCLAEL
541YQRKSREEST IAHQEDSKKK RGVPRTPVRQ KMNTMCRSLK MLNVARLNVK AQKLHPDGSP
601DVAGEKGIQK IPSGRTVDKL EDRGRTLRSS KPKDFKTEEE LLSYIRENYQ KTVATGEIML
661YACARNMIST VKMFLKSKGT KELEVNCLNQ VKSSLLKTSK SLRQNLGKKL DKEDKVRECQ
721LQVFLRLEMC LQCPSINEST DDMEQVVEEV TDLLRMVCLT EDSAYLAEFL EEILRLYIDS
781IPKTLGNLYN SLGFVIPQKL AGVLPTDFFS DDSMTQENKS PLLSVPFLSS ARRSVSGSPE
841SDELQELRTR SAKKRRKNAL IRHKSIAEVS QNLRQIEIPK VSKRATKKEN SHPAPQQPSQ
901PVKDTVQEVT KVRRNLFNQE LLSPSKRSLK RGLPRSHSVS AVDGLEDKLD NFKKNKGYHK
961LLTKSVAETP VHKQISKRLL HRQIKGRSSD PGPDIGVVEE SPEKGDEISL RRSPRIKQLS
1021FSRTHSASFY SVSQPKSRSV QRVHSFQQDK SDQRENSPVQ SIRSPKSLLF GAMSEMISPS
1081EKGSARMKKR SRNTLDSEVP AAYQTPKKSH QKSLSFSKTT PRRISHTPQT PLYTPERLQK
1141SPAKMTPTKQ AAFKESLKDS SSPGHDSPLD SKITPQKRHT QAGEGTSLET KTPRTPKRQG
1201TQPPGFLPNC TWPHSVNSSP ESPSCPAPPT SSTAQPRREC LTPIRDPLRT PPRAAAFMGT
1261PQNQTHQQPH VLRAARAEEP AQKLKDKAIK TPKRPGNSTV TSSPPVTPKK LFTSPLCDVS
1321KKSPFRKSKI ECPSPGELDQ KEPQMSPSVA ASLSCPVPST PPELSQRATL DTVPPPPPSK
1381VGKRCRKTSD PRRSIVECQP DASATPGVGT ADSPAAPTDS RDDQKGLSLS PQSPPERRGY
1441PGPGLRSDWH ASSPLLITSD TEHVTLLSEA EHHGIGDLKS NVLSVEEGEG LRTADAEKSS
1501LSHPGIPPSP PSCGPGSPLM PSCDVHCTTD GRQCQASAQL DNLPASAWHS TDSASPQTYE
1561VELEMQASGL PKLRIKKIDP SSSLEAEPLS KEESSLGEES FLPALSMPRA SRSLSKPEPT
1621YVSPPCPRLS HSTPGKSRGQ TYICQACTPT HGPSSTPSPF QTDGVPWTPS PKHSGKTTPD
1681IIKDWPRRKR AVGCGAGSSS GRGEVGADLP GSLSLLESEG KDHGLELSIH RTPILEDFEL
1741EGVCQLPDQS PPRNSMPKAE EASSWGQFGL SSRKRVLLAK EEADRGAKRI CDLREDSEVS
1801KSKEGSPSWS AWQLPSTGDE EVFVSGSTPP PSCAVRSCLS ASALQALTQS PLLFQGKTPS
1861SQSKDPRDED VDVLPSTVED SPFSRAFSRR RPISRTYTRK KLMGTWLEDL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1910 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.