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TIAM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TIAM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G121742-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TIAM2 (NM_001010927), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA2016 | STEF | TIAM-2 | TIAM2
Origin Species
Human
NCBI GenBank
AF120323
SwissProt
Q8IVF5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGPRENQDP PPRPLARHLS DADRLRKVIQ ELVDTEKSYV KDLSCLFELY LEPLQNETFL
61TQDEMESLFG SLPEMLEFQK VFLETLEDGI SASSDFNTLE TPSQFRKLLF SLGGSFLYYA
121DHFKLYSGFC ANHIKVQKVL ERAKTDKAFK AFLDARNPTK QHSSTLESYL IKPVQRVLKY
181PLLLKELVSL TDQESEEHYH LTEALKAMEK VASHINEMQK IYEDYGTVFD QLVAEQSGTE
241KEVTELSMGE LLMHSTVSWL NPFLSLGKAR KDLELTVFVF KRAVILVYKE NCKLKKKLPS
301NSRPAHNSTD LDPFKFRWLI PISALQVRLG NPAGTENNSI WELIHTKSEI EGRPETIFQL
361CCSDSESKTN IVKVIRSILR ENFRRHIKCE LPLEKTCKDR LVPLKNRVPV SAKLASSRSL
421KVLKNSSSNE WTGETGKGTL LDSDEGSLSS GTQSSGCPTA EGRQDSKSTS PGKYPHPGLA
481DFADNLIKES DILSDEDDDH RQTVKQGSPT KDIEIQFQRL RISEDPDVHP EAEQQPGPES
541GEGQKGGEQP KLVRGHFCPI KRKANSTKRD RGTLLKAQIR HQSLDSQSEN ATIDLNSVLE
601REFSVQSLTS VVSEECFYET ESHGKS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

626 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.