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THUMPD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

THUMPD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113204-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human THUMPD3 (NM_015453), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP434F091 | THUMP domain containing 3 | THUMPD3
Origin Species
Human
NCBI GenBank
NM_015453
SwissProt
Q9BV44

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCDIEEATNQ LLDVNLHENQ KSVQVTESDL GSESELLVTI GATVPTGFEQ TAADEVREKL
61GSSCKISRDR GKIYFVISVE SLAQVHCLRS VDNLFVVVQE FQDYQFKQTK EEVLKDFEDL
121AGKLPWSNPL KVWKINASFK KKKAKRKKIN QNSSKEKINN GQEVKIDQRN VKKEFTSHAL
181DSHILDYYEN PAIKEDVSTL IGDDLASCKD ETDESSKEET EPQVLKFRVT CNRAGEKHCF
241TSNEAARDFG GAVQDYFKWK ADMTNFDVEV LLNIHDNEVI VGIALTEESL HRRNITHFGP
301TTLRSTLAYG MLRLCDPLPY DIIVDPMCGT GAIPIEGATE WSDCFHIAGD NNPLAVNRAA
361NNIASLLTKS QIKEGKPSWG LPIDAVQWDI CNLPLRTGSV DIIVTDLPFG KRMGSKKRNW
421NLYPACLREM SRVCTPTTGR AVLLTQDTKC FTKALSGMRH VWRKVDTVWV NVGGLRAAVY
481VLIRTPQAFV HPSEQDGERG TLWQCKETRT RPLEQKLISE EDLAANDILD YKDDDDKV

538 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.