LSBio
THSD7A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

THSD7A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all THSD7A antibodies →
$725.00each
Catalog Number: LS-G113394-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human THSD7A (NM_015204), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_015204

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
185.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGLQARRWAS GSRGAAGPRR GVLQLLPLPL PLPLLLLLLL RPGAGRAAAQ GEAEAPTLYL
61WKTGPWGRCM GDECGPGGIQ TRAVWCAHVE GWTTLHTNCK QAERPNNQQN CFKVCDWHKE
121LYDWRLGPWN QCQPVISKSL EKPLECIKGE EGIQVREIAC IQKDKDIPAE DIICEYFEPK
181PLLEQACLIP CQQDCIVSEF SAWSECSKTC GSGLQHRTRH VVAPPQFGGS GCPNLTEFQV
241CQSSPCEAEE LRYSLHVGPW STCSMPHSRQ VRQARRRGKN KEREKDRSKG VKDPEARELI
301KKKRNRNRQN RQENKYWDIQ IGYQTREVMC INKTGKAADL SFCQQEKLPM TFQSCVITKE
361CQVSEWSEWS PCSKTCHDMV SPAGTRVRTR TIRQFPIGSE KECPEFEEKE PCLSQGDGVV
421PCATYGWRTT EWTECRVDPL LSQQDKRRGN QTALCGGGIQ TREVYCVQAN ENLLSQLSTH
481KNKEASKPMD LKLCTGPIPN TTQLCHIPCP TECEVSPWSA WGPCTYENCN DQQGKKGFKL
541RKRRITNEPT GGSGVTGNCP HLLEAIPCEE PACYDWKAVR LGNCEPDNGK ECGPGTQVQE
601VVCINSDGEE VDRQLCRDAI FPIPVACDAP CPKDCVLSTW STWSSCSHTC SGKTTEGKQI
661RARSILAYAG EEGGIRCPNS SALQEVRSCN EHPCTVYHWQ TGPWGQCIED TSVSSFNTTT
721TWNGEASCSV GMQTRKVICV RVNVGQVGPK KCPESLRPET VRPCLLPCKK DCIVTPYSDW
781TSCPSSCKEG DSSIRKQSRH RVIIQLPANG GRDCTDPLYE EKACEAPQAC QSYRWKTHKW
841RRCQLVPWSV QQDSPGAQEG CGPGRQARAI TCRKQDGGQA GIHECLQYAG PVPALTQACQ
901IPCQDDCQLT SWSKFSSCNG DCGAVRTRKR TLVGKSKKKE KCKNSHLYPL IETQYCPCDK
961YNAQPVGNWS DCILPEGKVE VLLGMKVQGD IKECGQGYRY QAMACYDQNG RLVETSRCNS
1021HGYIEEACII PCPSDCKLSE WSNWSRCSKS CGSGVKVRSK WLREKPYNGG RPCPKLDHVN
1081QAQVYEVVPC HSDCNQYLWV TEPWSICKVT FVNMRENCGE GVQTRKVRCM QNTADGPSEH
1141VEDYLCDPEE MPLGSRVCKL PCPEDCVISE WGPWTQCVLP CNQSSFRQRS ADPIRQPADE
1201GRSCPNAVEK EPCNLNKNCY HYDYNVTDWS TCQLSEKAVC GNGIKTRMLD CVRSDGKSVD
1261LKYCEALGLE KNWQMNTSCM VECPVNCQLS DWSPWSECSQ TCGLTGKMIR RRTVTQPFQG
1321DGRPCPSLMD QSKPCPVKPC YRWQYGQWSP CQVQEAQCGE GTRTRNISCV VSDGSADDFS
1381KVVDEEFCAD IELIIDGNKN MVLEESCSQP CPGDCYLKDW SSWSLCQLTC VNGEDLGFGG
1441IQVRSRPVII QELENQHLCP EQMLETKSCY DGQCYEYKWM ASAWKGSSRT VWCQRSDGIN
1501VTGGCLVMSQ PDADRSCNPP CSQPHSYCSE TKTCHCEEGY TEVMSSNSTL EQCTLIPVVV
1561LPTMEDKRGD VKTSRAVHPT QPSSNPAGRG RTWFLQPFGP DGRLKTWVYG VAAGAFVLLI
1621FIVSMIYLAC KKPKKPQRRQ NNRLKPLTLA YDGDADM
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1657 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.