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THSD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

THSD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all THSD4 antibodies →
$725.00each
Catalog Number: LS-G109728-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human THSD4 (NM_024817), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_024817
SwissProt
Q6ZMP0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
112.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVSHFMGSLS VLCFLLLLGF QFVCPQPSTQ HRKVPQRMAA EGAPEDDGGG GAPGVWGAWG
61PWSACSRSCS GGVMEQTRPC LPRSYRLRGG QRPGAPARAF ADHVVSAVRT SVPLHRSRDE
121TPALAGTDAS RQGPTVLRGS RHPQPQGLEV TGDRRSRTRG TIGPGKYGYG KAPYILPLQT
181DTAHTPQRLR RQKLSSRHSR SQGASSARHG YSSPAHQVPQ HGPLYQSDSG PRSGLQAAEA
241PIYQLPLTHD QGYPAASSLF HSPETSNNHG VGTHGATQSF SQPARSTAIS CIGAYRQYKL
301CNTNVCPESS RSIREVQCAS YNNKPFMGRF YEWEPFAEVK GNRKCELNCQ AMGYRFYVRQ
361AEKVIDGTPC DQNGTAICVS GQCKSIGCDD YLGSDKVVDK CGVCGGDNTG CQVVSGVFKH
421ALTSLGYHRV VEIPEGATKI NITEMYKSNN YLALRSRSGR SIINGNWAID RPGKYEGGGT
481MFTYKRPNEI SSTAGESFLA EGPTNEILDV YMIHQQPNPG VHYEYVIMGT NAISPQVPPH
541RRPGEPFNGQ MVTEGRSQEE GEQKGRNEEK EDLRGEAPEM FTSESAQTFP VRHPDRFSPH
601RPDNLVPPAP QPPRRSRDHN WKQLGTTECS TTCGKGSQYP IFRCVHRSTH EEAPESYCDS
661SMKPTPEEEP CNIFPCPAFW DIGEWSECSK TCGLGMQHRQ VLCRQVYANR SLTVQPYRCQ
721HLEKPETTST CQLKICSEWQ IRTDWTSCSV PCGVGQRTRD VKCVSNIGDV VDDEECNMKL
781RPNDIENCDM GPCAKSWFLT EWSERCSAEC GAGVRTRSVV CMTNHVSSLP LEGCGNNRPA
841EATPCDNGPC TGKVEWFAGS WSQCSIECGS GTQQREVICV RKNADTFEVL DPSECSFLEK
901PPSQQSCHLK PCGAKWFSTE WSMCSKSCQG GFRVREVRCL SDDMTLSNLC DPQLKPEERE
961SCNPQDCVPE VDENCKDKYY NCNVVVQARL CVYNYYKTAC CASCTRVANR QTGFLGSR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1018 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.