Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
THSD1 / TMTSP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111659-100
Catalog Number: LS-G111659
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human THSD1 (NM_018676), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- UNQ3010 | THSD1 | TMTSP
- Origin Species
- Human
- NCBI GenBank
- NM_018676
- SwissProt
- Q9NS62
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 94.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKPMLKDFSN LLLVVLCDYV LGEAEYLLLR EPGHVALSND TVYVDFQYFD GANGTLRNVS61VLLLEANTNQ TVTTKYLLTN QSQGTLKFEC FYFKEAGDYW FTMTPEATDN STPFPWWEKS121AFLKVEWPVF HVDLNRSAKA AEGTFQVGLF TSQPLCPFPV DKPNIVVDVI FTNSLPEARR181NSRQPLEIRT SKRTELAQGQ WVEFGCAPLG PEAYVTVVLK LLGRDSVITS TGPIDLAQKF241GYKLVMVPEL TCESGVEVTV LPPPCTFVQG VVTVFKEAPR YPGKRTIHLA ENSLPLGERR301TIFNCTLFDM GKNKYCFDFG ISSRSHFSAK EECMLIQRNT ETWGLWQPWS QCSATCGDGV361RERRRVCLTS FPSSPVCPGM SLEASLCSLE ECAAFQPSSP SPLQPQGPVK SNNIVTVTGI421SLCLFIIIAT VLITLWRRFG RPAKCSTPAR HNSIHSPSFR KNSDEENICE LSEQRGSFSD481GGDGPTGSPG DTGIPLTYRR SGPVPPEDDA SGSESFQSNA QKIIPPLFSY RLAQQQLKEM541KKKGLTETTK VYHVSQSPLT DTAIDAAPSA PLDLESPEEA AANKFRIKSP FPEQPAVSAG601ERPPSRLDLN VTQASCAISP SQTLIRKSQA RHVGSRGGPS ERSHARNAHF RRTASFHEAR661QARPFRERSM STLTPRQAPA YSSRTRTCEQ AEDRFRPQSR GAHLFPEKLE HFQEASGTRG721PLNPLPKSYT LGQPLRKPDL GDHQAGLVAG IERTEPHRAR RGPSPSHKSV SRKQSSPISP781KDNYQRVSSL SPSQCRKDKC QSFPTHPEFA FYDNTSFGLT EAEQRMLDLP GYFGSNEEDE841TTSTLSVEKL VITRTRPLEQKLISEEDLAANDILDYKDDDDKV
852 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.