Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
THPO / TPO / Thrombopoietin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129664-100
Catalog Number: LS-G129664
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Thrombopoietin(THPO) (NM_001177597), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C-mpl ligand | MGDF | MPL ligand | THCYT1 | Thrombopoietin nirs variant 1 | MKCSF | THPO | MPLLG | Thrombopoietin
- Origin Species
- Human
- NCBI GenBank
- NM_000460
- SwissProt
- P40225
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MELTELLLVV MLLLTARLTL SSPAPPACDL RVLSKLLRDS HVLHSRLSQC PEVHPLPTPV61LLPAVDFSLG EWKTQMEETK AQDILGAVTL LLEGVMAARG QLGPTCLSSL LGQLSGQVRL121LLGALQSLLG TQGRTTAHKD PNAIFLSFQH LLRGKVRFLM LVGGSTLCVR RAPPTTAVPS181RTSLVLTLNE LPNRTSGLLE TNFTASARTT GSGLLKWQQG FRAKIPGLLN QTSRSLDQIP241GYLNRIHELL NGTRGLFPGP SRRTLGAPDI SSGTSDTGSL PPNLQPGYSP SPTHPPTGQY301TLFPLPPTLP TPVVQLHPLL PDPSAPTPTP TSPLLNTSYT HSQNLSQEGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
349 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.