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THNSL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

THNSL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all THNSL1 antibodies →
$504.00each
Catalog Number: LS-G109673-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human THNSL1 (NM_024838), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Threonine synthase-like 1 | TSH1 | THNSL1
Origin Species
Human
NCBI GenBank
NM_024838
SwissProt
Q8IYQ7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLHFNRCHHL KKITQKCFSS IHVKTDKHAQ RFLSRTFALA ELRKSWYSTH SLVGDKNIIL
61MGPPGAGKTT VGRIIGQKLG CCVIDVDDDI LEKTWNMSVS EKLQDVGNEQ FLEEEGKAVL
121NFSASGSVIS LTGSNPMHDA SMWHLKKNGI IVYLDVPLLD LICRLKLMKT DRIVGQNSGT
181SMKDLLKFRR QYYKKWYDAR VFCESGASPE EVADKVLNAI KRYQDVDSET FISTRHVWPE
241DCEQKVSAKF FSEAVIEGLA SDGGLFVPAK EFPKLSCGEW KSLVGATYVE RAQILLERCI
301HPADIPAARL GEMIETAYGE NFACSKIAPV RHLSGNQFIL ELFHGPTGSF KDLSLQLMPH
361IFAHCIPPSC NYMILVATSG DTGSAVLNGF SRLNKNDKQR IAVVAFFPEN GVSDFQKAQI
421IGSQRENGWA VGVESDFDFC QTAIKRIFND SDFTGFLTVE YGTILSSANS INWGRLLPQV
481VYHASAYLDL VSQGFISFGS PVDVCIPTGN FGNILAAVYA KMMGIPIRKF ICASNQNHVL
541TDFIKTGHYD LRERKLAQTF SPSIDILKSS NLERHLHLMA NKDGQLMTEL FNRLESQHHF
601QIEKALVEKL QQDFVADWCS EGECLAAINS TYNTSGYILD PHTAVAKVVA DRVQDKTCPV
661IISSTAHYSK FAPAIMQALK IKEINETSSS QLYLLGSYNA LPPLHEALLE RTKQQEKMEY
721QVCAADMNVL KSHVEQLVQN QFI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

743 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.