LSBio
THAP7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

THAP7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all THAP7 antibodies →
$504.00each
Catalog Number: LS-G121852-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human THAP7 (NM_001008695), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
THAP7 | THAP domain containing 7
Origin Species
Human
NCBI GenBank
NM_030573
SwissProt
Q9BT49

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
34.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPRHCSAAGC CTRDTRETRN RGISFHRLPK KDNPRRGLWL ANCQRLDPSG QGLWDPASEY
61IYFCSKHFEE DCFELVGISG YHRLKEGAVP TIFESFSKLR RTTKTKGHSY PPGPAEVSRL
121RRCRKRCSEG RGPTTPFSPP PPADVTCFPV EEASAPATLP ASPAGRLEPG LSSPFSDLLG
181PLGAQADEAG CSAQPSPERQ PSPLEPRPVS PSAYMLRLPP PAGAYIQNEH SYQVGSALLW
241KRRAEAALDA LDKAQRQLQA CKRREQRLRL RLTKLQQERA REKRAQADAR QTLKEHVQDF
301AMQLSSSMA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

309 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.