Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TGM7 / Transglutaminase 7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108022-100
Catalog Number: LS-G108022
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Transglutaminase 7(TGM7) (NM_052955), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TG(Z) | TGase Z | TGase-7 | Transglutaminase Z | TGMZ | TGZ | Transglutaminase-7 | TGM7 | Transglutaminase 7
- Origin Species
- Human
- NCBI GenBank
- AI018564
- SwissProt
- Q96PF1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 79.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDQVATLRLE SVDLQSSRNN KEHHTQEMGV KRLTVRRGQP FYLRLSFSRP FQSQNDHITF61VAETGPKPSE LLGTRATFFL TRVQPGNVWS ASDFTIDSNS LQVSLFTPAN AVIGHYTLKI121EISQGQGHSV TYPLGTFILL FNPWSPEDDV YLPSEILLQE YIMRDYGFVY KGHERFITSW181PWNYGQFEED IIDICFEILN KSLYHLKNPA KDCSQRNDVV YVCRVVSAMI NSNDDNGVLQ241GNWGEDYSKG VSPLEWKGSV AILQQWSARG GQPVKYGQCW VFASVMCTVM RCLGVPTRVV301SNFRSAHNVD RNLTIDTYYD RNAEMLSTQK RDKIWNFHVW NECWMIRKDL PPGYNGWQVL361DPTPQQTSSG LFCCGPASVK AIREGDVHLA YDTPFVYAEV NADEVIWLLG DGQAQEILAH421NTSSIGKEIS TKMVGSDQRQ SITSSYKYPE GSPEERAVFM KASRKMLGPQ RASLPFLDLL481ESGGLRDQPA QLQLHLARIP EWGQDLQLLL RIQRVPDSTH PRGPIGLVVR FCAQALLHGG541GTQKPFWRHT VRMNLDFGKE TQWPLLLPYS NYRNKLTDEK LIRVSGIAEV EETGRSMLVL601KDICLEPPHL SIEVSERAEV GKALRVHVTL TNTLMVALSS CTMVLEGSGL INGQIAKDLG661TLVAGHTLQI QLDLYPTKAG PRQLQVLISS NEVKEIKGYK DIFVTVAGAPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
710 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.