Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TGM5 / Transglutaminase 5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116774-100
Catalog Number: LS-G116774
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Transglutaminase 5(TGM5) (NM_004245), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TG(X) | TGase-5 | Transglutaminase 5 | TGase X | TGASE5 | TGASEX | TGMX | TGX | Transglutaminase V | Transglutaminase X | Transglutaminase-5 | TGM5
- Origin Species
- Human
- NCBI GenBank
- NM_004245
- SwissProt
- O43548
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQGLEVALT DLQSSRNNVR HHTEEITVDH LLVRRGQAFN LTLYFRNRSF QPGLDNIIFV61VETEDAVYLD SEPQRQEYVM NDYGFIYQGS KNWIRPCPWN YGQFEDKIID ICLKLLDKSL121HFQTDPATDC ALRGSPVYVS RVVCAMINSN DDNGVLNGNW SENYTDGANP AEWTGSVAIL181KQWNATGCQP VRYGQCWVFA AVMCTVMRCL GIPTRVITNF DSGHDTDGNL IIDEYYDNTG241RILGNKKKDT IWNFHVWNEC WMARKDLPPA YGGWQVLDAT PQEMSNGVYC CGPASVRAIK301EGEVDLNYDT PFVFSMVNAD CMSWLVQGGK EQKLHQDTSS VGNFISTKSI QSDERDDITE361NYKYEEGSLQ ERQVFLKALQ KLKARSFHGS QRGAELQPSR PTSLSQDSPR SLHTPSLRPS421DVVQVSLKFK LLDPPNMGQD ICFVLLALNM SSQFKDLKVN LSAQSLLHDG SPLSPFWQDT481AFITLSPKEA KTYPCKISYS QYSQYLSTDK LIRISALGEE KSSPEKILVN KIITLSYPSI541TINVLGAAVV NQPLSIQVIF SNPLSEQVED CVLTVEGSGL FKKQQKVFLG VLKPQHQASI601ILETVPFKSG QRQIQANMRS NKFKDIKGYR NVYVDFALTRTRPLEQKLISEEDLAANDILDYKDDDDKV
638 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.