Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TGM4 / Transglutaminase 4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117473-100
Catalog Number: LS-G117473
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Transglutaminase 4(TGM4) (NM_003241), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Fibrinoligase | HTGP | TGase-4 | Transglutaminase-4 | Prostate transglutaminase | TGase P | TGP | Transglutaminase P | TG(P) | TGM4 | Transglutaminase 4 (prostate)
- Origin Species
- Human
- NCBI GenBank
- NM_003241
- SwissProt
- P49221
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 77 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MMDASKELQV LHIDFLNQDN AVSHHTWEFQ TSSPVFRRGQ VFHLRLVLNQ PLQSYHQLKL61EFSTGPNPSI AKHTLVVLDP RTPSDHYNWQ ATLQNESGKE VTVAVTSSPN AILGKYQLNV121KTGNHILKSE ENILYLLFNP WCKEDMVFMP DEDERKEYIL NDTGCHYVGA ARSIKCKPWN181FGQFEKNVLD CCISLLTESS LKPTDRRDPV LVCRAMCAMM SFEKGQGVLI GNWTGDYEGG241TAPYKWTGSA PILQQYYNTK QAVCFGQCWV FAGILTTVLR ALGIPARSVT GFDSAHDTER301NLTVDTYVNE NGEKITSMTH DSVWNFHVWT DAWMKRPDLP KGYDGWQAVD ATSQERSQGV361FCCGPSPLTA IRKGDIFIVY DTRFVFSEVN GDRLIWLVKM VNGQEELHVI SMETTSIGKN421ISTKAVGQDR RRDITYEYKY PEGSSEERQV MDHAFLLLSS EREHRRPVKE NFLHMSVQSD481DVLLGNSVNF TVILKRKTAA LQNVNILGSF ELQLYTGKKM AKLCDLNKTS QIQGQVSEVT541LTLDSKTYIN SLAILDDEPV IRGFIIAEIV ESKEIMASEV FTSFQYPEFS IELPNTGRIG601QLLVCNCIFK NTLAIPLTDV KFSLESLGIS SLQTSDHGTV QPGETIQSQI KCTPIKTGPK661KFIVKLSSKQ VKEINAQKIV LITKTRTRPL EQKLISEEDL AANDILDYKD DDDKV
715 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.