TG737 / IFT88 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115245-100
Catalog Number: LS-G115245
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC10(IFT88) (NM_006531), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DAF19 | D13S1056E | HTg737 | RP11-172H24.2 | Polaris homolog | TPR repeat protein 10 | TTC10 | IFT88 | TG737
- Origin Species
- Human
- NCBI GenBank
- NM_006531
- SwissProt
- Q13099
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 93 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MMQNVHLAPE TDEDDLYSGY NDYNPIYDIE ELENDAAFQQ AVRTSHGRRP PITAKISSTA61VTRPIATGYG SKTSLASSIG RPMTGAIQDG VTRPMTAVRA AGFTKAALRG SAFDPLSQSR121GPASPLEAKK KDSPEEKIKQ LEKEVNELVE ESCIANSCGD LKLALEKAKD AGRKERVLVR181QREQVTTPEN INLDLTYSVL FNLASQYSVN EMYAEALNTY QVIVKNKMFS NAGILKMNMG241NIYLKQRNYS KAIKFYRMAL DQVPSVNKQM RIKIMQNIGV TFIQAGQYSD AINSYEHIMS301MAPNLKAGYN LTICYFAIGD REKMKKAFQK LITVPLEIDE DKYISPSDDP HTNLVTEAIK361NDHLRQMERE RKAMAEKYIM TSAKLIAPVI ETSFAAGYDW CVEVVKASQY VELANDLEIN421KAVTYLRQKD YNQAVEILKV LEKKDSRVKS AAATNLSALY YMGKDFAQAS SYADIAVNSD481RYNPAALTNK GNTVFANGDY EKAAEFYKEA LRNDSSCTEA LYNIGLTYEK LNRLDEALDC541FLKLHAILRN SAEVLYQIAN IYELMENPSQ AIEWLMQVVS VIPTDPQVLS KLGELYDREG601DKSQAFQYYY ESYRYFPCNI EVIEWLGAYY IDTQFWEKAI QYFERASLIQ PTQVKWQLMV661ASCFRRSGNY QKALDTYKDT HRKFPENVEC LRFLVRLCTD LGLKDAQEYA RKLKRLEKMK721EIREQRIKSG RDGSGGSRGK REGSASGDSG QNYSASSKGE RLSARLRALP GTNEPYESSS781NKEIDASYVD PLGPQIERPK TAAKKRIDED DFADEELGDD LLPETRTRPLEQKLISEEDLAANDILDYKDDDDKV
824 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.