Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TFDP2 / DP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129781-100
Catalog Number: LS-G129781
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DP2(TFDP2) (NM_001178140), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DP2 | E2F dimerization partner 2 | Dp-2 | TFDP2 | Transcription factor Dp-2
- Origin Species
- Human
- NCBI GenBank
- U18422
- SwissProt
- Q14188
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQPEGIIFEA ENKPSPGTES AGTFILDLSA TSRTIISTPQ RLTSSGSVLI GSPYTPAPAM61VTQTHIAEAT GWVPGDRKRA RKFIDSDFSE SKRSKKGDKN GKGLRHFSMK VCEKVQRKGT121TSYNEVADEL VSEFTNSNNH LAADSAYDQK NIRRRVYDAL NVLMAMNIIS KEKKEIKWIG181LPTNSAQECQ NLEIEKQRRI ERIKQKRAQL QELLLQQIAF KNLVQRNRQN EQQNQGPPAL241NSTIQLPFII INTSRKTVID CSISSDKFEY LFNFDNTFEI HDDIEVLKRM GMSFGLESGK301CSLEDLKLAK SLVPKALEGY ITDISTGPSW LNQGLLLNST QSVSNLDLTT GATLPQSSVN361QGLCLDAEVA LATGQFLAPN SHQSSSAASH CSESRGETPC SFNDEDEEDD EEDSSSPETRTRPLEQKLISEEDLAANDILDYKDDDDKV
418 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.