Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TFCP2 / CP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129841-100
Catalog Number: LS-G129841
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CP2c(TFCP2) (NM_001173453), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CP2 | LSF1D | LSF | LBP-1C | SAA3 enhancer factor | SEF | TFCP2C | TFCP2 | Transcription factor CP2 | LBP1C | Transcription factor LSF
- Origin Species
- Human
- NCBI GenBank
- NM_005653
- SwissProt
- Q12800
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAWALKLPLA DEVIESGLVQ DFDASLSGIG QELGAGAYSM SDVLALPIFK QEESSLPPDN61ENKILPFQYV LCAATSPAVK LHDETLTYLN QGQSYEIRML DNRKLGELPE INGKLVKSIF121RVVFHDRRLQ YTEHQQLEGW RWNRPGDRIL DIDIPMSVGI IDPRANPTQL NTVEFLWDPA181KRTSVFIQPK GADRKQKTDR EKMEKRTPHE KEKYQPSYET TILTECSPWP EITYVNNSPS241PGFNSSHSSF SLGEGNGSPN HQPEPPPPVT DNLLPTTTPQ EAQQWLHRNR FSTFTRLFTN301FSGADLLKLT RDDVIQICGP ADGIRLFNAL KGRMVRPRLT IYVCQESLQL REQQQQQQQQ361QQKHEDGDSN GTFFVYHAIY LEELTAVELT EKIAQLFSIS PCQISQIYKQ GPTGIHVLIS421DEMIQNFQEE ACFILDTMKE TNDSYHIILKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
450 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.