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TEX10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TEX10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TEX10 antibodies →
$504.00each
Catalog Number: LS-G112246-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TEX10 (NM_017746), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA208F1.2 | RP11-208F1.2 | Testis expressed gene 10 | Testis expressed sequence 10 | Ipi1 | Testis expressed 10 | TEX10
Origin Species
Human
NCBI GenBank
NM_017746

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
105.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTKKRKRQHD FQKVKLKVGK KKPKLQNATP TNFKTKTIHL PEQLKEDGTL PTNNRKLNIK
61DLLSQMHHYN AGVKQSALLG LKDLLSQYPF IIDAHLSNIL SEVTAVFTDK DANVRLAAVQ
121LLQFLAPKIR AEQISPFFPL VSAHLSSAMT HITEGIQEDS LKVLDILLEQ YPALITGRSS
181ILLKNFVELI SHQQLSKGLI NRDRSQSWIL SVNPNRRLTS QQWRLKVLVR LSKFLQALAD
241GSSRLRESEG LQEQKENPHA TSNSIFINWK EHANDQQHIQ VYENGGSQPN VSSQFRLRYL
301VGGLSGVDEG LSSTENLKGF IEIIIPLLIE CWVEAVPPQL ATPVGNGIER EPLQVMQQVL
361NIISLLWKLS KQQDETHKLE SWLRKNYLID FKHHFMSRFP YVLKEITKHK RKEPNKSIKH
421CTVLSNNIDH LLLNLTLSDI MVSLANASTL QKDCSWIEMI RKFVTETLED GSRLNSKQLN
481RLLGVSWRLM QIQPNREDTE TLIKAVYTLY QQRGLILPVR TLLLKLFSKI YQTEELRSCR
541FRYRSKVLSR WLAGLPLQLA HLGSRNPELS TQLIDIIHTA AARANKELLK SLQATALRIY
601DPQEGAVVVL PADSQQRLVQ LVYFLPSLPA DLLSRLSRCC IMGRLSSSLA AMLIGILHMR
661SSFSGWKYSA KDWLMSDVDY FSFLFSTLTG FSKEELTWLQ SLRGVPHVIQ TQLSPVLLYL
721TDLDQFLHHW DVTEAVFHSL LVIPARSQNF DILQSAISKH LVGLTVIPDS TAGCVFGVIC
781KLLDHTCVVS ETLLPFLASC CYSLLYFLLT IEKGEAEHLR KRDKLWGVCV SILALLPRVL
841RLMLQSLRVN RVGPEELPVV GQLLRLLLQH APLRTHMLTN AILVQQIIKN ITTLKSGSVQ
901EQWLTDLHYC FNVYITGHPQ GPSALATVY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

929 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.