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TESK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TESK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G114807-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TESK2 (NM_007170), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TESK2 | Testicular protein kinase 2 | Testis-specific kinase 2
Origin Species
Human
NCBI GenBank
NM_007170
SwissProt
Q96S53

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDRSKRNSIA GFPPRVERLE EFEGGGGGEG NVSQVGRVWP SSYRALISAF SRLTRLDDFT
61CEKIGSGFFS EVFKVRHRAS GQVMALKMNT LSSNRANMLK EVQLMNRLSH PNILRFMGVC
121VHQGQLHALT EYINSGNLEQ LLDSNLHLPW TVRVKLAYDI AVGLSYLHFK GIFHRDLTSK
181NCLIKRDENG YSAVVADFGL AEKIPDVSMG SEKLAVVGSP FWMAPEVLRD EPYNEKADVF
241SYGIILCEII ARIQADPDYL PRTENFGLDY DAFQHMVGDC PPDFLQLTFN CCNMDPKLRP
301SFVEIGKTLE EILSRLQEEE QERDRKLQPT ARGLLEKAPG VKRLSSLDDK IPHKSPCPRR
361TIWLSRSQSD IFSRKPPRTV SVLDPYYRPR DGAARTPKVN PFSARQDLMG GKIKFFDLPS
421KSVISLVFDL DAPGPGTMPL ADWQEPLAPP IRRWRSLPGS PEFLHQEACP FVGREESLSD
481GPPPRLSSLK YRVKEIPPFR ASALPAAQAH EAMDCSILQE ENGFGSRPQG TSPCPAGASE
541EMEVEERPAG STPATFSTSG IGLQTQGKQD G
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

571 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.