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TEM7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TEM7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PLXDC1 antibodies →
$504.00each
Catalog Number: LS-G101668-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TEM7(PLXDC1) (NM_020405), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PLXDC1 | TEM3 | Tumor endothelial marker 3 | Tumor endothelial marker 7 | TEM7 | Plexin domain containing 1
Origin Species
Human
NCBI GenBank
NM_020405
SwissProt
Q8IUK5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MRGELWLLVL VLREAARALS PQPGAGHDEG PGSGWAAKGT VRGWNRRARE SPGHVSEPDR
61TQLSQDLGGG TLAMDTLPDN RTRVVEDNHS YYVSRLYGPS EPHSRELWVD VAEANRSQVK
121IHTILSNTHR QASRVVLSFD FPFYGHPLRQ ITMATGGFIF MGDVIHRMLT ATQYVAPLMA
181NFNPGYSDNS TVVYFDNGTV FVVQWDHVYL QGWEDKGSFT FQAALHHDGR IVFAYKEIPM
241SVPEISSSQH PVKTGLSDAF MILNPSPDVP ESRRRSIFEY HRIELDPSKV TSMSAVEFTP
301LPTCLQHRSC DACMSSDLTF NCSWCHVLQR CSSGFDRYRQ EWMDYGCAQE AEGRMCEDFQ
361DEDHDSASPD TSFSPYDGDL TTTSSSLFID SLTTEDDTKL NPYAGGDGLQ NNLSPKTKGT
421PVHLGTIVGI VLAVLLVAAI ILAGIYINGH PTSNAALFFI ERRPHHWPAM KFRSHPDHST
481YAEVEPSGHE KEGFMEAEQC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

500 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.