Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TEL-2 / ETV7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112837-100
Catalog Number: LS-G112837
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ETV7 (NM_016135), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ETS translocation variant 7 | Ets variant 7 | ETV7 | TELB | TEL2 oncogene | Transcription factor ETV7 | TEL-2 | TEL2 | TREF | ETS-related protein Tel2 | Tel-related Ets factor | Transcription factor Tel-2
- Origin Species
- Human
- NCBI GenBank
- NM_016135
- SwissProt
- Q9Y603
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQEGELAISP ISPVAAMPPL GTHVQARCEA QINLLGEGGI CKLPGRLRIQ PALWSREDVL61HWLRWAEQEY SLPCTAEHGF EMNGRALCIL TKDDFRHRAP SSGDVLYELL QYIKTQRRAL121VCGPFFGGIF RLKTPTQHSP VPPEEVTGPS QMDTRRGHLL QPPDPGLTSN FGHLDDPGLA181RWTPGKEESL NLCHCAELGC RTQGVCSFPA MPQAPIDGRI ADCRLLWDYV YQLLLDTRYE241PYIKWEDKDA KIFRVVDPNG LARLWGNHKN RVNMTYEKMS RALRHYYKLN IIKKEPGQKL301LFRFLKTPGK MVQDKHSHLE PLESQEQDRI EFKDKRPEIS PTRTRPLEQKLISEEDLAANDILDYKDDDDKV
341 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.