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TEKT5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TEKT5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TEKT5 antibodies →
$725.00each
Catalog Number: LS-G106926-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TEKT5 (NM_144674), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Tektin-5 | TEKT5 | Tektin 5
Origin Species
Human
SwissProt
Q96M29

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MEFLGTTQTA SYCGPKKCCG LTSLPAVQAP VIQECYQPYY LPGYRYLNSW RPSLFYKIAN
61VQTCPDESTS TLRPPTILPT LRSALFSRYS PHDWDQSNQL QVRGAEASRL WASRLTDDSM
121RLLQDKDQLT HQMQEGTCRN LGQRLSDIGF WKSELSYELD RLLTENQNLE TVKRRLECAA
181NEVNCPLQVA LECLYHREKR IGIDLVHDNV EKNLIREVDL LKCCQEQMRK LAQRIDIQMR
241DNRDAQHVLE RDLEDKSSAQ CIDEKCFNLR NTSDCISFFH GMEKIDGTIS VPETWAKFSN
301DNIKHSQNMR ANSIQLREEA EHLFETLSDQ MWRQFTDTNL AFNARISEVT DVKNKLQTQL
361AKTLQEIFQA ENTIMLLERS IMAKEGPLKV AQTRLECRTR RPNMELCRDI PQLKLVNEVF
421TIDDTLQTLK LRLRETQDTL QLLVMTKCRL EHELAIKANT LCIDKEKCMG MRKTFPCTPR
481LVGHT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

485 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.