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TDRKH Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TDRKH Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G115033-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TDRKH (NM_006862), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TDRD2 | TDRKH | Tudor domain containing 2 | Tudor and KH domain containing | Putative RNA binding protein
Origin Species
Human
NCBI GenBank
NM_006862
SwissProt
Q9Y2W6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.02 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSTERTSWTS LSTIQKIALG LGIPASATVA YILYRRYRES REERLTFVGE DDIEIEMRVP
61QEAVKLIIGR QGANIKQLRK QTGARIDVDT EDVGDERVLL ISGFPVQVCK AKAAIHQILT
121ENTPVSEQLS VPQRSVGRII GRGGETIRSI CKASGAKITC DKESEGTLLL SRLIKISGTQ
181KEVAAAKHLI LEKVSEDEEL RKRIAHSAET RVPRKQPISV RREDMTEPGG AGEPALWKNT
241SSSMEPTAPL VTPPPKGGGD MAVVVSKEGS WEKPSDDSFQ KSEAQAIPEM PMFEIPSPDF
301SFHADEYLEV YVSASEHPNH FWIQIVGSRS LQLDKLVNEM TQHYENSVPE DLTVHVGDIV
361AAPLPTNGSW YRARVLGTLE NGNLDLYFVD FGDNGDCPLK DLRALRSDFL SLPFQAIECS
421LARIAPSGDQ WEEEALDEFD RLTHCADWKP LVAKISSYVQ TGISTWPKIY LYDTSNGKKL
481DIGLELVHKG YAIELPEDIE ENRAVPDMLK DMATETDASL STLLTETKKS SGEITHTLSC
541LSLSEAASMS GDDNLEDDYL LGSLGFSLLS HLLCCMLPAM NSLKHAQPWN WCYQSSVGNL
601YSLEVP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

606 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.