LSBio
TDRD6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TDRD6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TDRD6 antibodies →
$725.00each
Catalog Number: LS-G121704-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TDRD6 (NM_001010870), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA446F17.4 | CT41.2 | NY-CO-45 | TDR2 | Tudor domain containing 6 | Tudor repeat 2 | Antigen NY-CO-45 | TDRD6 | Cancer/testis antigen 41.2 | SPATA36
Origin Species
Human
NCBI GenBank
AF039442
SwissProt
O60522

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
236.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCSTPGMPAP GASLALRVSF VDVHPDVIPV QLWGLVGERR GEYLRLSREI QEAAATRGQW
61ALGSASASPG ELCLVQVGLL WHRCRVVSRQ AQESRVFLLD EGRTITAGAG SLAPGRREFF
121NLPSEVLGCV LAGLVPAGCG AGSGEPPQHW PADAVDFLSN LQGKEVHGCV LDVLLLHRLV
181LLEVPDVFQQ MRELGLARRV PDSLFRSLLE RYLTAATASV GSGVPVLSRV PLKQKQPGLD
241YFYPQLQLGV TEAVVITQVC HPHRIHCQLR SVSQEIHRLS ESMAQVYRGS TGTGDENSTS
301ATWEEREESP DKPGSPCASC GLDGHWYRAL LLETFRPQRC AQVLHVDYGR KELVSCSSLR
361YLLPEYFRMP VVTYPCALYG LWDGGRGWSR SQVGDLKTLI LGKAVNAKIE FYCSFEHVYY
421VSLYGEDGIN LNRVFGVQSC CLADRVLQSQ ATEEEEPETS QSQSPAEEVD EEISLPALRS
481IRLKMNAFYD AQVEFVKNPS EFWIRLRKHN VTFSKLMRRM CGFYSSASKL DGVVLKPEPD
541DLCCVKWKEN GYYRAIVTKL DDKSVDVFLV DRGNSENVDW YDVRMLLPQF RQLPILAVKC
601TLADIWPLGK TWSQEAVSFF KKTVLHKELV IHILDKQDHQ YVIEILDESR TGEENISKVI
661AQAGYAKYQE FETKENILVN AHSPGHVSNH FTTESNKIPF AKTGEGEQKA KRENKTTSVS
721KALSDTTVVT NGSTELVVQE KVKRASVYFP LMQNCLEIKP GSSSKGELEV GSTVEVRVSY
781VENPGYFWCQ LTRNIQGLKT LMSDIQYYCK NTAAPHQRNT LACLAKRTVN RQWSRALISG
841IQSVEHVNVT FVDYGDREMV SVKNIYSISE EFLKVKAQAF RCSLYNLIQP VGQNPFVWDV
901KAIQAFNEFI DNAWQKNLEL KCTIFALASI NEELFNIVDL LTPFQSACHF LVEKRLARPV
961KLQKPLESSV QLHSYFYSTH DMKIGSEELV YITHIDDPWT FYCQLARNAN ILEQLSCSIT
1021QLSKVLLNLK TSPLNPGTLC LAKYTDGNWY RGIVIEKEPK KVFFVDFGNI YVVTSDDLLP
1081IPSDAYDVLL LPMQAVRCSL SDIPDHIPEE VVVWFQETIL DKSLKALVVA KDPDGTLIIE
1141LYGDNIQISA SINKKLGLLS YKDRIRKKES EVLCSTTETL EEKNENMKLP CTEYLSKSVG
1201YKLPNKEILE ESYKPQINSS YKELKLLQSL TKTNLVTQYQ DSVGNKNSQV FPLTTEKKEE
1261ISAETPLKTA RVEATLSERK IGDSCDKDLP LKFCEFPQKT IMPGFKTTVY VSHINDLSDF
1321YVQLIEDEAE ISHLSERLNS VKTRPEYYVG PPLQRGDMIC AVFPEDNLWY RAVIKEQQPN
1381DLLSVQFIDY GNVSVVHTNK IGRLDLVNAI LPGLCIHCSL QGFEVPDNKN SKKMMHYFSQ
1441RTSEAAIRCE FVKFQDRWEV ILADEHGIIA DDMISRYALS EKSQVELSTQ VIKSASSKSV
1501NKSDIDTSVF LNWYNPEKKM IRAYATVIDG PEYFWCQFAD TEKLQCLEVE VQTAGEQVAD
1561RRNCIPCPYI GDPCIVRYRE DGHYYRALIT NICEDYLVSV RLVDFGNIED CVDPKALWAI
1621PSELLSVPMQ AFPCCLSGFN ISEGLCSQEG NDYFYEIITE DVLEITILEI RRDVCDIPLA
1681IVDLKSKGKS INEKMEKYSK TGIKSALPYE NIDSEIKQTL GSYNLDVGLK KLSNKAVQNK
1741IYMEQQTDEL AEITEKDVNI IGTKPSNFRD PKTDNICEGF ENPCKDKIDT EELEGELECH
1801LVDKAEFDDK YLITGFNTLL PHANETKEIL ELNSLEVPLS PDDESKEFLE LESIELQNSL
1861VVDEEKGELS PVPPNVPLSQ ECVTKGAMEL FTLQLPLSCE AEKQPELELP TAQLPLDDKM
1921DPLSLGVSQK AQESMCTEDM RKSSCVESFD DQRRMSLHLH GADCDPKTQN EMNICEEEFV
1981EYKNRDAISA LMPLFSEEES SDGSKHNNGL PDHISAQLQN TYTLKAFTVG SKCVVWSSLR
2041NTWSKCEILE TAEEGTRVLN LSNGMEEIVN PENVWNGIPK LDKSPPEKRG LEVMEI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

2096 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.