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TDRD5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TDRD5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TDRD5 antibodies →
$725.00each
Catalog Number: LS-G105186-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TDRD5 (NM_173533), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001199085

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSEQERIQEC LRKEIRSLLI STKDGLSPQE LEKEYLLMVG NHLPLRILGY RSTMELVLDM
61PDVVRVCPGA GGTVILKAIP DESTKGIASL VAKQRSSHKL RNSMHKGRPS IYSGPRSHRR
121VPYRGRVAPI LPAVVKSELK DLLALSPVLL SDFEKAFAKR FGRSFQYMQY GFLSMFEVLN
181AASDVISVEQ TRAGSLLMLK KSVTEEKPRG CPAGKIFTQP FRMKQGSYST GFPVAKPCFS
241QPTSNMEPPK QIMSMEKTSK LNVVETSRLN HTEKLNQLEN TFKSVIAQIG PGGTISSELK
301HKIKFVVSKF PEGLFISKLL GEYEVIFKEQ LSPKKLGFLN VTELVGALSD ILHVEFRKGH
361QDLLVFDADK KPLPPVQSDK KIEAKACVSS PPRNSLSTAA VKETVWNCPS KKQKEPQQKI
421CKKPNLVVKP LQLQVETNKS ELNLAMANHD IPPDAVPNKK LCRLPPLDTS SLIGVFVEYI
481ISPSQFYIRI YSRDSSELLE DMMIEMRRCY SNQLVSDRYV MPECFIQPGH LCCVRISEDK
541WWYRVIIHRV LEKQEVEVFY PDFGNIGIVQ KSSLRFLKCC YTKLPAQAIP CSLAWVRPVE
601EHWTSKAILQ FQKLCGLKPL VGVVDEYVDG ILNIFLCDTS SNEDVYFHHV LRTEGHAIVC
661RENISSKGFS ELNPLALYTT SSGGPEDIVL TELGYPSQQH YFNEDRKISP QSKESELRIL
721DEIPTGMPCL ESVTIGDDIW DENWLPLQAK MGKGGDAASH LFTASLGGKN QYSSCKEMPQ
781KDWCFSTPKD TWDDSWQPSG LVNGTKVEVH KPEVLGAQEK NTGTNRTQKQ LDINGSSDSS
841TLPKLEEFCT SLTQSEQSAD GSQSEPNNSQ TQPKQIQLST AAPCSTTAVD DSAEKPSGSV
901ESSPEILKNE DFSSSRAITL YKDKRQESVD QLSLILSYEC QISQKLYIPR STATAALGAA
961ARLATSRSLL HWYPSVKRME A
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

981 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.