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TCTN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TCTN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G121555-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TCTN3 (NM_001013840), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP564D116 | OFD4 | TECT3 | Tectonic 3 | Tectonic-3 | C10orf61 | JBTS18 | TCTN3 | Tectonic family member 3
Origin Species
Human
NCBI GenBank
AL050022
SwissProt
Q6NUS6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRLETAEAYV GPGGPECPMR TPQLALLQVF FLVFPDGVRP QPSSSPSGAV PTSLELQRGT
61DGGTLQSPSE ATATRPAVPG LPTVVPTLVT PSAPGNRTVD LFPVLPICVC DLTPGACDIN
121CCCDRDCYLL HPRTVFSFCL PGSVRSSSWV CVDNSVIFRS NSPFPSRVFM DSNGIRQFCV
181HVNNSNLNYF QKLQKVNATN FQALAAEFGG ESFTSTFQTQ SPPSFYRAGD PILTYFPKWS
241VISLLRQPAG VGAGGLCAES NPAGFLESKS TTCTRFFKNL ASSCTLDSAL NAASYYNFTV
301LKVPRSMTDP QNMEFQVPVI LTSQANAPLL AGNTCQNVVS QVTYEIETNG TFGIQKVSVS
361LGQTNLTVEP GASLQQHFIL RFRAFQQSTA ASLTSPRSGN PGYIVGKPLL ALTDDISYSM
421TLLQSQGNGS CSVKRHEVQF GVNAISGCKL RLKKADCSHL QQEIYQTLHG RPRPEYVAIF
481GNADPAQKGG WTRILNRHCS ISAINCTSCC LIPVSLEIQV LWAYVGLLSN PQAHVSGVRF
541LYQCQSIQDS QQVTEVSLTT LVNFVDITQK PQPPRGQPKM DWKWPFDFFP FKVAFSRGVF
601SQKCSVSPIL ILCLLLLGVL NLETM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

625 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.