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TCTN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TCTN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109936-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TCTN1 (NM_024549), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
JBTS13 | TECT1 | Tectonic-1 | TCTN1 | Tectonic family member 1
Origin Species
Human
NCBI GenBank
NM_024549
SwissProt
Q2MV58

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRPRGLPPLL VVLLGCWASV SAQTDATPAV TTEGLNSTEA ALATFGTFPS TRPPGTPRAP
61GPSSGPRPTP VTDVAVLCVC DLSPAQCDIN CCCDPDCSSV DFSVFSACSV PVVTGDSQFC
121SQKAVIYSLN FTANPPQRVF ELVDQINPSI FCIHITNYKP ALSFINPEVP DENNFDTLMK
181TSDGFTLNAE SYVSFTTKLD IPTAAKYEYG VPLQTSDSFL RFPSSLTSSL CTDNNPAGQA
241YWFTPVIPAL WEAEARGSLE VPDSRKKVPI TVQSIVIQSL NKTLTRREDT DVLQPTLVNA
301GHFSLCVNVV LEVKYSLTYT DAGEVTKADL SFVLGTVSSV VVPLQQKFEI HFLQENTQPV
361PLSGNPGYVV GLPLAAGFQP HKGSGIIQTT NRYGQLTILH STTEQDCLAL EGVRTPVLFG
421YTMQSGCKLR LTGALPCQLV AQKVKSLLWG QGFPDYVAPF GNSQAQDMLD WVPIHFITQS
481FNRKDSCQLP GALVIEVKWT KYGSLLNPQA KIVNVTANLI SSSFPEANSG NERTILISTA
541VTFVDVSAPA EAGFRAPPAI NARLPFNFFF PFV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

573 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.