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TCP11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TCP11 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TCP11 antibodies →
$725.00each
Catalog Number: LS-G119307-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TCP11 (NM_001093728), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
D6S230E | KIAA0229 | T-complex protein 11 homolog | FPPR | T-complex 11 homolog (mouse) | T-complex 11 | testis-specific | TCP11
Origin Species
Human
NCBI GenBank
NM_018679
SwissProt
Q8WWU5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTRGGGGGDT ISKMPDVKES VPPKYPGDSE GRSCKPETSG PPQEDKSGSE DPPPFLSVTG
61LTETVNEVSK LSNKIGMNCD YYMEEKVLPP SSLEGKVKET VHNAFWDHLK EQLSATPPDF
121SCALELLKEI KEILLSLLLP RQNRLRIEIE EALDMDLLKQ EAEHGALKVL YLSKYVLNMM
181ALLCAPVRDE AVQKLENITD PVWLLRGIFQ VLGRMKMDMV NYTIQSLQPH LQEHSIQYER
241AKFQELLNKQ PSLLNHTTKW LTQAAGDLTM SPPTCPDTSD SSSVAGPSPN EAANNPEPLS
301PTMVLCQGFL NLLLWDLENE EFPETLLMDR TRLQELKSQL HQLTVMASVL LVASSFSGSV
361LFGSPQFVDK LKRITKSLLE DFHSRPEEAI LTVSEQVSQE IHQSLKNMGL VALSSDNTAS
421LMGQLQNIAK KENCVCSVID QRIHLFLKCC LVLGVQRSLL DLPGGLTLIE AELAELGQKF
481VNLTHHNQQV FGPYYTEILK TLISPAQALE TKVESV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

516 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.