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TCN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TCN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TCN1 antibodies →
$725.00each
Catalog Number: LS-G99318-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TCN1 (NM_001062), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Haptocorin | Haptocorrin | TC I | TCI | Transcobalamin I | TC-1 | TC1 | TCN1 | Transcobalamin-1
NCBI GenBank
NM_001062
SwissProt
P20061

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MRQSHQLPLV GLLLFSFIPS QLCEICEVSE ENYIRLKPLL NTMIQSNYNR GTSAVNVVLS
61LKLVGIQIQT LMQKMIQQIK YNVKSRLSDV SSGELALIIL ALGVCRNAEE NLIYDYHLID
121KLENKFQAEI ENMEAHNGTP LTNYYQLSLD VLALCLFNGN YSTAEVVNHF TPENKNYYFG
181SQFSVDTGAM AVLALTCVKK SLINGQIKAD EGSLKNISIY TKSLVEKILS EKKENGLIGN
241TFSTGEAMQA LFVSSDYYNE NDWNCQQTLN TVLTEISQGA FSNPNAAAQV LPALMGKTFL
301DINKDSSCVS ASGNFNISAD EPITVTPPDS QSYISVNYSV RINETYFTNV TVLNGSVFLS
361VMEKAQKMND TIFGFTMEER SWGPYITCIQ GLCANNNDRT YWELLSGGEP LSQGAGSYVV
421RNG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

423 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.