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TCHHL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TCHHL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G121326-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TCHHL1 (NM_001008536), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Protein S100-A17 | S100A17 | TCHHL1 | THHL1 | Trichohyalin-like 1 | Trichohyalin-like protein 1 | Basalin
Origin Species
Human
SwissProt
Q5QJ38

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPQLLRNVLC VIETFHKYAS EDSNGATLTG RELKQLIQGE FGDFFQPCVL HAVEKNSNLL
61NIDSNGIISF DEFVLAIFNL LNLCYLDIKS LLSSELRQVT KPEKEKLDDV DVQATTGDGQ
121WTVGTSPTQE KRMLPSGMAS SSQLIPEESG AVGNNRVDPW REAKTHNFPG EASEHNDPKN
181KHLEGDEQSQ EVAQDIQTTE DNEGQLKTNK PMAGSKKTSS PTERKGQDKE ISQEGDEPAR
241EQSVSKIRDQ FGEQEGNLAT QSSPPKEATQ RPCEDQEVRT EKEKHSNIQE PPLQREDEPS
301SQHADLPEQA AARSPSQTQK STDSKDVCRM FDTQEPGKDA DQTPAKTKNL GEPEDYGRTS
361ETQEKECETK DLPVQYGSRN GSETSDMRDE RKERRGPEAH GTAGQKERDR KTRPLVLETQ
421TQDGKYQELQ GLSKSKDAEK GSETQYLSSE GGDQTHPELE GTAVSGEEAE HTKEGTAEAF
481VNSKNAPAAE RTLGARERTQ DLAPLEKQSV GENTRVTKTH DQPVEEEDGY QGEDPESPFT
541QSDEGSSETP NSLASEEGNS SSETGELPVQ GDSQSQGDQH GESVQGGHNN NPDTQRQGTP
601GEKNRALEAV VPAVRGEDVQ LTEDQEQPAR GEHKNQGPGT KGPGAAVEPN GHPEAQESTA
661GDENRKSLEI EITGALDEDF TDQLSLMQLP GKGDSRNELK VQGPSSKEEK GRATEAQNTL
721LESLDEDNSA SLKIQLETKE PVTSEEEDES PQELAGEGGD QKSPAKKEHN SSVPWSSLEK
781QMQRDQEPCS VERGAVYSSP LYQYLQEKIL QQTNVTQEEH QKQVQIAQAS GPELCSVSLT
841SEISDCSVFF NYSQASQPYT RGLPLDESPA GAQETPAPQA LEDKQGHPQR ERLVLQREAS
901TTKQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

904 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.