Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TCF7L2 / TCG4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128182-100
Catalog Number: LS-G128182
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TCF7L2 (NM_001146283), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HMG box transcription factor 4 | HTCF-4 | T-cell factor-4 variant X2 | T-cell factor 4 | Transcription factor 7-like 2 | TCF-4 | TCF7L2
- Origin Species
- Human
- NCBI GenBank
- NM_030756
- SwissProt
- Q9NQB0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPQLNGGGGD DLGANDELIS FKDEGEQEEK SSENSSAERD LADVKSSLVN ESETNQNSSS61DSEAERRPPP RSESFRDKSR ESLEEAAKRQ DGGLFKGPPY PGYPFIMIPD LTSPYLPNGS121LSPTARTYLQ MKWPLLDVQA GSLQSRQALK DARSPSPAHI VSPLPCCTQG HDCQHFYPPS181DFTVSTQVFR DMKRSHSLQK VGEPWCIESN KVPVVQHPHH VHPLTPLITY SNEHFTPGNP241PPHLPADVDP KTGIPRPPHP PDISPYYPLS PGTVGQIPHP LGWLVPQQGQ PVYPITTGGF301RHPYPTALTV NASMSRFPPH MVPPHHTLHT TGIPHPAIVT PTVKQESSQS DVGSLHSSKH361QDSKKEEEKK KPHIKKPLNA FMLYMKEMRA KVVAECTLKE SAAINQILGR RWHALSREEQ421AKYYELARKE RQLHMQLYPG WSARDNYGKK KKRKRDKQPG ETNGEKKSAF ATYKVKAAAS481AHPLQMEAYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
489 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.