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TCF25 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TCF25 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TCF25 antibodies →
$504.00each
Catalog Number: LS-G113536-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Transcription factor 25(TCF25) (NM_014972), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FKSG26 | Hulp1 | Nuclear localized protein 1 | NULP1 | PRO2620 | TCF-25 | HKIAA1049 | KIAA1049 | TCF25 | Transcription factor 25
Origin Species
Human
NCBI GenBank
NM_014972
SwissProt
Q9BQ70

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSRRALRRLR GEQRGQEPLG PGALHFDLRD DDDAEEEGPK RELGVRRPGG AGKEGVRVNN
61RFELINIDDL EDDPVVNGER SGCALTDAVA PGNKGRGQRG NTESKTDGDD TETVPSEQSH
121ASGKLRKKKK KQKNKKSSTG EASENGLEDI DRILERIEDS TGLNRPGPAP LSSRKHVLYV
181EHRHLNPDTE LKRYFGARAI LGEQRPRQRQ RVYPKCTWLT TPKSTWPRYS KPGLSMRLLE
241SKKGLSFFAF EHSEEYQQAQ HKFLVAVESM EPNNIVVLLQ TSPYHVDSLL QLSDACRFQE
301DQEMARDLVE RALYSMECAF HPLFSLTSGA CRLDYRRPEN RSFYLALYKQ MSFLEKRGCP
361RTALEYCKLI LSLEPDEDPL CMLLLIDHLA LRARNYEYLI RLFQEWEAHR NLSQLPNFAF
421SVPLAYFLLS QQTDLPECEQ SSARQKASLL IQQALTMFPG VLLPLLESCS VRPDASVSSH
481RFFGPNAEIS QPPALSQLVN LYLGRSHFLW KEPATMSWLE ENVHEVLQAV DAGDPAVEAC
541ENRRKVLYQR APRNIHRHVI LSEIKEAVAA LPPDVTTQSV MGFDPLPPSD TIYSYVRPER
601LSPISHGNTI ALFFRSLLPN YTMEGERPEE GVAGGLNRNQ GLNRLMLAVR DMMANFHLND
661LEAPHEDDAE GEGEWD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

676 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.