Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TCF19 / TCF-19 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120026-100
Catalog Number: LS-G120026
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TCF19 (NM_001077511), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SC1(TCF19)-7 | SC1 | TCF-19 | TCF19 | Transcription factor 19 | Transcription factor SC1 | SCI(TCF19)-4 | SC1(TCF19)-6 | SC1-1
- Origin Species
- Human
- NCBI GenBank
- NM_007109
- SwissProt
- Q9Y242
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLPCFQLLRI GGGRGGDLYT FHPPAGAGCT YRLGHRADLC DVALRPQQEP GLISGIHAEL61HAEPRGDDWR VSLEDHSSQG TLVNNVRLPR GHRLELSDGD LLTFGPEGPP GTSPSEFYFM121FQQVRVKPQD FAAITIPRSR GEARVGAGFR PMLPSQGAPQ RPLSTFSPAP KATLILNSIG181SLSKLRPQPL TFSPSWGGPK SLPVPAPPGE VGTTPSAPPQ RNRRKSVHRV LAELDDESEP241LENPPPVLME PRKKLRVDKA PLTPTGNRRG RPRKYPVSAP MAPPAVGGGE PCAAPCCCLP301QEETVAWVQC DGCDVWFHVA CVGCSIQAAR EADFRCPGCR AGIQTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
345 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.