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TCERG1L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TCERG1L Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TCERG1L antibodies →
$504.00each
Catalog Number: LS-G105079-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TCERG1L (NM_174937), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TCERG1L
Origin Species
Human
SwissProt
Q5VWI1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVPGSAGLLR LSAGVVVPPV LLASAPPPAA PLLPGLPGWP APSEPVLPLL PLPSAPDSAA
61AAAAHPFPAL HGQWLFGGHS PSLGLPPSST VELVPVFPHL CPSALATPIG KSWIDKRIPN
121CKIFFNNSFA LDSTWIHPEE SRFFHGHEKP RLLANQVAVS LSRPAPASRP LPTVVLAPQP
181IPGGCHNSLK VTSSPAIAIA TAAAAAMVSV DPENLRGPSP SSVQPRHFLT LAPIKIPLRT
241SPVSDTRTER GRVARPPALM LRAQKSRDGD KEDKEPPPML GGGEDSTARG NRPVASTQVP
301GSPWCVVWTG DDRVFFFNPT MHLSVWEKPM DLKDRGDLNR IIEDPPHKRK LEAPATDNSD
361GSSSEDNRED QDVKTKRNRT EGCGSPKPEE AKREDKGTRT PPPQILLPLE ERVTHFRDML
421LERGVSAFST WEKELHKIVF DPRYLLLNSE ERKQIFEQFV KTRIKEEYKE KKSKLLLAKE
481EFKKLLEKSK VSPRTTFKEF AEKYGRDQRF RLVQKRKDQE HFFNKFILIL KKRDKENRLR
541LRKMR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

545 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.