Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TCERG1 / CA150 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120432-100
Catalog Number: LS-G120432
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CA150(TCERG1) (NM_001040006), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CA150 | Co-activator of 150 kDa | TCERG1 | TAF2S | Transcription factor CA150 | Urn1
- Origin Species
- Human
- NCBI GenBank
- NM_006706
- SwissProt
- O14776
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 121.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAERGGDGGE SERFNPGELR MAQQQALRFR GPAPPPNAVM RGPPPLMRPP PPFGMMRGPP61PPPRPPFGRP PFDPNMPPMP PPGGIPPPMG PPHLQRPPFM PPPMSSMPPP PGMMFPPGMP121PVTAPGTPAL PPTEEIWVEN KTPDGKVYYY NARTRESAWT KPDGVKVIQQ SELTPMLAAQ181AQVQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ241AQVQAQVQAQ VQAQAVGAST PTTSSPAPAV STSTSSSTPS STTSTTTTAT SVAQTVSTPT301TQDQTPSSAV SVATPTVSVS TPAPTATPVQ TVPQPHPQTL PPAVPHSVPQ PTTAIPAFPP361VMVPPFRVPL PGMPIPLPGV LPGMAPPIVP MIHPQVAIAA SPATLAGATA VSEWTEYKTA421DGKTYYYNNR TLESTWEKPQ ELKEKEKLEE KIKEPIKEPS EEPLPMETEE EDPKEEPIKE481IKEEPKEEEM TEEEKAAQKA KPVATAPIPG TPWCVVWTGD ERVFFYNPTT RLSMWDRPDD541LIGRADVDKI IQEPPHKKGM EELKKLRHPT PTMLSIQKWQ FSMSAIKEEQ ELMEEINEDE601PVKAKKRKRD DNKDIDSEKE AAMEAEIKAA RERAIVPLEA RMKQFKDMLL ERGVSAFSTW661EKELHKIVFD PRYLLLNPKE RKQVFDQYVK TRAEEERREK KNKIMQAKED FKKMMEEAKF721NPRATFSEFA AKHAKDSRFK AIEKMKDREA LFNEFVAAAR KKEKEDSKTR GEKIKSDFFE781LLSNHHLDSQ SRWSKVKDKV ESDPRYKAVD SSSMREDLFK QYIEKIAKNL DSEKEKELER841QARIEASLRE REREVQKARS EQTKEIDRER EQHKREEAIQ NFKALLSDMV RSSDVSWSDT901RRTLRKDHRW ESGSLLEREE KEKLFNEHIE ALTKKKREHF RQLLDETSAI TLTSTWKEVK961KIIKEDPRCI KFSSSDRKKQ REFEEYIRDK YITAKADFRT LLKETKFITY RSKKLIQESD1021QHLKDVEKIL QNDKRYLVLD CVPEERRKLI VAYVDDLDRR GPPPPPTASE PTRRSTKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1077 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.