LSBio
TCEB3CL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TCEB3CL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TCEB3CL antibodies →
$725.00each
Catalog Number: LS-G118939-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TCEB3CL (NM_001100817), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001100817

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAGSTTLRA VGKLQVRLAT KTEPKKLEKY LQKLSALPMT ADILAETGIR KTVKRLRKHQ
61HVGDFARDLA ARWKKLVLVD RNTGPDPQDP EESASRQRFG EALQEREKAW GFPENATAPR
121SPSHSPEHRR TARRTPPGQQ RPHPRSPSRE PRAERKRPRM APADSGPHRD PPTRTAPLPM
181PEGPEPAVPG EQPGRGHAHA AQGGPLLGQG CQGQPQGEAV GSHSKGHKSS RGASAQKSPP
241VQESQSERLQ AAGADSAGPK TVPSHVFSEL WDPSEAWMQA NYDLLSAFEA MTSQANPEAL
301SAPTLQEEAA FPGRRVNAKM PVYSGSRPAC QLQVPTLRQQ CLRVPRNNPD ALGDVEGVPY
361SVLEPVLEGW TPDQPYRTEK DNAALARETD ELWRIHCLQD FKEEKPQEHE SWRELYLRLR
421DAREQRLRVV TTKIRSAREN KPSGRQTKMI CFNSVAKTPY DASRRQEKSA GAADPGNGEM
481EPAPKPAGSS QAPSGLGDGD GGSVSGGGSS NRHAAPADKT RKQAAKKVAP LMAKAIRDYK
541GRFSRR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

546 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.