Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TCEB3C / Elongin A3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G106628-100
Catalog Number: LS-G106628
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TCEB3C (NM_145653), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Elongin-A3 | TCEB3C | EloA3 | HsT829 | TCEB3L2
- Origin Species
- Human
- SwissProt
- Q8NG57
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAGSTTLRA VGKLQVRLAT KTEPKKLEKY LQKLSALPMT ADILAETGIR KTVKRLRKHQ61HVGDFARDLA ARWKKLVLVD RNTGPDPQDP EESASRQRFG EALQEREKAW GFPENATAPR121SPSHSPEHRR TARRTPPGQQ RPHPRSPSRE PRAERKRPRM APADSGPHRD PPTRTAPLPM181PEGPEPAVPG EQPGRGHAHA AQGGPLLGQG CQGQPQGEAV GSHSKGHKSS RGASAQKSPP241VQESQSERLQ AAGADSAGPK TVPSHVFSEL WDPSEAWMQA NYDLLSAFEA MTSQANPEAL301SAPTLQEEAA FPGRRVNAKM PVYSGSRPAC QLQVPTLRQQ CLRVPRNNPD ALGDVEGVPY361SVLEPVLEGW TPDQPYRTEK DNAALARETD ELWRIHCLQD FKEEKPQEHE SWRELYLRLR421DAREQRLRVV TTKIRSAREN KPSGRQTKMI CFNSVAKTPY DASRRQEKSA GAADPGNGEM481EPAPKPAGSS QAPSGLGDGD GGSVSGGGSS NRHAAPADKT RKQAAKKVAP LMAKAIRDYK541GRFSRRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
546 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.