Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TCEB3B / Elongin A2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112687-100
Catalog Number: LS-G112687
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TCEB3B (NM_016427), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ELOA2 | TCEB3L | TCEB3B | Elongin-A2 | HsT832
- Origin Species
- Human
- NCBI GenBank
- NM_016427
- SwissProt
- Q8IYF1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 83.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAGSTTLHA VEKLQVRLAT KTEPKKLEKY LQKLSALPMT ADILAETGIR KTVKRLRKHQ61HVGDFARDLA ARWKKLVLVD RNTRPGPQDP EESASRQRFG EALQDQEKAW GFPENATAPR121SPSHSPEHRR TARRTPPGQQ RPHPRSHSRE PRAERKCPRI APADSGRYRA SPTRTAPLRM181PEGPEPAAPG KQPGRGHTHA AQGGPLLCPG CQGQPQGKAV VSHSKGHKSS RQEKRPLCAQ241GDWHSPTLIR EKSCGACLRE ETPRMPSWAS ARDRQPSDFK TDKEGGQAGS GQRVPALEEA301PDSHQKRPQH SHSNKKRPSL DGRDPGNGTH GLSPEEKEQL SNDRETQEGK PPTAHLDRTS361VSSLSEVEEV DMAEEFEQPT LSCEKYLTYD QLRKQKKKTG KSATTALGDK QRKANESKGT421RESWDSAKKL PPVQESQSER LQAAGADSAG PKTVPNHVFS ELWDLSEAWM QANYDPLSDS481DSMTSQAKPE ALSSPKFREE AAFPGRRVNA KMPVYSGSRP ACQLQVPTLR QQCAQVLRNN541PDALSDVGEV PYWVLEPVLE GWRPDQLYRR KKDNHALVRE TDELRRNHCF QDFKEEKPQE601NKTWREQYLR LPDAPEQRLR VMTTNIRSAR GNNPNGREAK MICFKSVAKT PYDTSRRQEK661SAGDADPENG EIKPASKPAG SSHTPSSQSS SGGGRDSSSS ILRWLPEKRA NPCLSSSNEH721AAPAAKTRKQ AAKKVAPLMA KAIRDYKRRF SRRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
753 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.