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TCAIM Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TCAIM Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TCAIM antibodies →
$725.00each
Catalog Number: LS-G105168-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C3orf23(TCAIM) (NM_173826), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C3orf23 | TOAG-1 | TOAG1 | TCAIM | Tolerance associated gene-1 | D9Ertd402e
Origin Species
Human
SwissProt
Q8N3R3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFCHLRPMRR LCLEKIFPHW FPFSRALSGA EAVNALRPFY FAVHPDFFGQ HPVEREINEN
61SLKRLSVYLE NLQKPGFKSL KPTQLTFYVR ETDQSSSDGQ EPFSTSGFRA VKFTLHTRDL
121LSTVLYILNS CSLSVEHIQS LNTNMHTQPL KEAKRMPDRP IKWDKSYYSF TGFKDPDEDL
181EQVSRVETTL TSWLDNNGKS AVKKLKNSLP LRKELDRLKD ELSHQLQLSD IRWQRSWGIA
241HRCSQLHSLS RLAQQNLETL KKAKGCTIIF TDRSGMSAVG HVMLGTMDVH HHWTKLFERL
301PSYFDLQRRL MILEDQISYL LGGIQVVYIE ELQPVLTLEE YYSLLDVFYN RLLKSRILFH
361PRSLRGLQMI LNSDRYAPSL HELGHFNIPT LCDPANLQWF ILTKAQQARE NMKRKEELKV
421IENELIQAST KKFSLEKLYK EPSISSIQMV DCCKRLLEQS LPYLHGMHLC ISHFYSVMQD
481GDLCIPWNWK NGEAIK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

496 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.