Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TCAF2 / FAM115C Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105286-100
Catalog Number: LS-G105286
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM115C(TCAF2) (NM_173678), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FAM115C | FAM139A | Protein FAM139A | Protein FAM115C
- Origin Species
- Human
- SwissProt
- A6NFQ2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 91.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATIAAAAFE ALMDGVTCWD VPRGPIPSEL LLIGEAAFPV MVNDKGQVLI AASSYGRGRL61VVVSHEGYLS HTGLAPFLLN AVSWLCPCPG APVGVHPSLA PLVNILQDAG LEAQVKPEPG121EPLGVYCINA YNDTLTATLI QFVKHGGGLL IGGQAWYWAS QHGPDKVLSR FPGNKVTSVA181GVYFTDTYGD RDRFKVSKKV PKIPLHVRYG EDVRQDQQQL LEGISELDIR TGGVPSQLLV241HGALAFPLGL DASLNCFLAA AHYGRGRVVL AAHECLLCAP KMGPFLLNAV RWLARGQTGK301VGVNTNLKDL CPLLSEHGLQ CSLEPHLNSD LCVYCCKAYS DKEAKQLQEF VAEGGGLLIG361GQAWWWASQN PGHCPLAGFP GNIILNCFGL SILPQTLKAG CFPVPTPEMR SYHFRKALSQ421FQAILNHENG NLEKSCLAKL RVDGAAFLQI PAEGVPAYIS LHRLLRKMLR GSGLPAVSRE481NPVASDSYEA AVLSLATGLA HSGTDCSQLA QGLGTWTCSS SLYPSKHPIT VEINGINPGN541NDCWVSTGLY LLEGQNAEVS LSEAAASAGL RVQIGCHTDD LTKARKLSRA PVVTHQCWMD601RTERSVSCLW GGLLYVIVPK GSQLGPVPVT IRGAVPAPYY KLGKTSLEEW KRQMQENLAP661WGELATDNII LTVPTTNLQA LKDPEPVLRL WDEMMQAVAR LAAEPFPFRR PERIVADVQI721SAGWMHSGYP IMCHLESVKE IINEMDMRSR GVWGPIHELG HNQQRHGWEF PPHTTEATCN781LWSVYVHETV LGIPRAQAHE ALSPPERERR IKAHLGKGAP LCDWNVWTAL ETYLQVLSRN841SGRRGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
845 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.