Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TCAF1 / FAM115A Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113759-100
Catalog Number: LS-G113759
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human F115A(TCAF1) (NM_014719), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FAM115A | KIAA0738 | Protein FAM115A
- Origin Species
- Human
- SwissProt
- Q9Y4C2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 102 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATPSAAFEA LMNGVTSWDV PEDAVPCELL LIGEASFPVM VNDMGQVLIA ASSYGRGRLV61VVSHEDYLVE AQLTPFLLNA VGWLCSSPGA PIGVHPSLAP LAKILEGSGV DAKVEPEVKD121SLGVYCIDAY NETMTEKLVK FMKCGGGLLI GGQAWDWANQ GEDERVLFTF PGNLVTSVAG181IYFTDNKGDT SFFKVSKKMP KIPVLVSCED DLSDDREELL HGISELDISN SDCFPSQLLV241HGALAFPLGL DSYHGCVIAA ARYGRGRVVV TGHKVLFTVG KLGPFLLNAV RWLDGGRRGK301IVVQTELRTL SGLLAVGGID TSIEPNLTSD ASVYCFEPVS EVGVKELQEF VAEGGGLFVG361AQAWWWAFKN PGVSPLARFP GNLLLNPFGI SITSQSLNPG PFRTPKAGIR TYHFRSTLAE421FQVIMGRKRG NVEKGWLAKL GPDGAAFLQI PAEEIPAYMS VHRLLRKLLS RYRLPVATRE481NPVINDCCRG AMLSLATGLA HSGSDLSLLV PEIEDMYSSP YLRPSESPIT VEVNCTNPGT541RYCWMSTGLY IPGRQIIEVS LPEAAASADL KIQIGCHTDD LTRASKLFRG PLVINRCCLD601KPTKSITCLW GGLLYIIVPQ NSKLGSVPVT VKGAVHAPYY KLGETTLEEW KRRIQENPGP661WGELATDNII LTVPTANLRT LENPEPLLRL WDEVMQAVAR LGAEPFPLRL PQRIVADVQI721SVGWMHAGYP IMCHLESVQE LINEKLIRTK GLWGPVHELG RNQQRQEWEF PPHTTEATCN781LWCVYVHETV LGIPRSRANI ALWPPVREKR VRIYLSKGPN VKNWNAWTAL ETYLQLQEAF841GWEPFIRLFT EYRNQTNLPT ENVDKMNLWV KMFSHQVQKN LAPFFEAWAW PIQKEVATSL901AYLPEWKENI MKLYLLTQMP HTRTRPLEQKLISEEDLAANDILDYKDDDDKV
921 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.