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TC2N Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TC2N Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TC2N antibodies →
$504.00each
Catalog Number: LS-G102347-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TC2N (NM_152332), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf47 | MTAC2D1 | Tandem C2 protein in nucleus | TC2N | C2CD1 | Tac2-N | Tandem C2 domains | nuclear
Origin Species
Human
NCBI GenBank
NM_152332
SwissProt
Q8N9U0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATEFIKSCC GGCFYGETEK HNFSVERDFK AAVPNSQNAT ISVPPLTSVS VKPQLGCTED
61YLLSKLPSDG KEVPFVVPKF KLSYIQPRTQ ETPSHLEELE GSARASFGDR KVELSSSSQH
121GPSYDVYNPF YMYQHISPDL SRRFPPRSEV TRLYGSVCDL RTNKLPGSPG LSKSMFDLTN
181SSQRFIQRHD SLSSVPSSSS SRKNSQGSNR SLDTITLSGD ERDFGRLNVK LFYNSSVEQI
241WITVLQCRDL SWPSSYGDTP TVSIKGILTL PKPVHFKSSA KEGSNAIEFM ETFVFAIKLQ
301NLQTVRLVFK IQTQTPRKKT IGECSMSLRT LSTQEMDYSL DITPPSKISV CHAELELGTC
361FQAVNSRIQL QILEARYLPS SSTPLTLSFF VKVGMFSSGE LIYKKKTRLL KASNGRVKWG
421ETMIFPLIQS EKEIVFLIKL YSRSSVRRKH FVGQIWISED SNNIEAVNQW KETVINPEKV
481VIRWHKLNPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

490 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.