LSBio
TBX22 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBX22 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TBX22 antibodies →
$504.00each
Catalog Number: LS-G112475-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBX22 (NM_016954), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLPA | DJ795G23.1 | CPX | TBXX | T-box 22 | T-box protein 22 | TBX22 | TBOX22
Origin Species
Human
NCBI GenBank
NM_016954
SwissProt
Q9Y458

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALSSRARAF SVEALVGRPS KRKLQDPIQA EQPELREKKG GEEEEERRSS AAGKSEPLEK
61QPKTEPSTSA SSGCGSDSGY GNSSESLEEK DIQMELQGSE LWKRFHDIGT EMIITKAGRR
121MFPSVRVKVK GLDPGKQYHV AIDVVPVDSK RYRYVYHSSQ WMVAGNTDHL CIIPRFYVHP
181DSPCSGETWM RQIISFDRMK LTNNEMDDKG HIILQSMHKY KPRVHVIEQG SSVDLSQIQS
241LPTEGVKTFS FKETEFTTVT AYQNQQITKL KIERNPFAKG FRDTGRNRGV LDGLLETYPW
301RPSFTLDFKT FGADTQSGSS GSSPVTSSGG APSPLNSLLS PLCFSPMFHL PTSSLGMPCP
361EAYLPNVNLP LCYKICPTNF WQQQPLVLPA PERLASSNSS QSLAPLMMEV PMLSSLGVTN
421SKSGSSEDSS DQYLQAPNST NQMLYGLQSP GNIFLPNSIT PEALSCSFHP SYDFYRYNFS
481MPSRLISGSN HLKVNDDSQV SFGEGKCNHV HWYPAINHYL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

520 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.