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TBX15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBX15 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TBX15 antibodies →
$725.00each
Catalog Number: LS-G106349-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBX15(TBX15) (NM_152380), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TBX14 | TBX15 | T-box 14 | T-box 15 | T-box protein 15 | T-box protein 14
Origin Species
Human
NCBI GenBank
NM_152380
SwissProt
Q96SF7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSMEEIQVE LQCADLWKRF HDIGTEMIIT KAGRRMFPAM RVKITGLDPH QQYYIAMDIV
61PVDNKRYRYV YHSSKWMVAG NADSPVPPRV YIHPDSLASG DTWMRQVVSF DKLKLTNNEL
121DDQGHIILHS MHKYQPRVHV IRKDFSSDLS PTKPVPVGDG VKTFNFPETV FTTVTAYQNQ
181QITRLKIDRN PFAKGFRDSG RNRTGLEAIM ETYAFWRPPV RTLTFEDFTT MQKQQGGSTG
241TSPTTSSTGT PSPSASSHLL SPSCSPPTFH LAPNTFNVGC RESQLCNLNL SDYPPCARSN
301MAALQSYPGL SDSGYNRLQS GTTSATQPSE TFMPQRTPSL ISGIPTPPSL PGNSKMEAYG
361GQLGSFPTSQ FQYVMQAGNA ASSSSSPHMF GGSHMQQSSY NAFSLHNPYN LYGYNFPTSP
421RLAASPEKLS ASQSTLLCSS PSNGAFGERQ YLPSGMEHSM HMISPSPNNQ QATNTCDGRQ
481YGAVPGSSSQ MSVHMV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

496 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.