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TBR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G100857-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBR1 (NM_006593), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TBR-1 | TBR1 | T-box brain protein 1 | T-brain-1 | T-box | brain | 1 | TES-56
Origin Species
Human
NCBI GenBank
NM_006593
SwissProt
Q16650

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQLEHCLSPS IMLSKKFLNV SSSYPHSGGS ELVLHDHPII STTDNLERSS PLKKITRGMT
61NQSDTDNFPD SKDSPGDVQR SKLSPVLDGV SELRHSFDGS AADRYLLSQS SQPQSAATAP
121SAMFPYPGQH XXPAHPAFSI GSPSRYMAHH PVITNGAYNS LLSNSSPQGY PTAGYPYPQQ
181YGHSYQGAPF YQFSSTQPGL VPGKAQVYLC NRPLWLKFHR HQTEMIITKQ GRRMFPFLSF
241NISGLDPTAH YNIFVDVILA DPNHWRFQGG KWVPCGKADT NVQGNRVYMH PDSPNTGAHW
301MRQEISFGKL KLTNNKGASN NNGQMVVLQS LHKYQPRLHV VEVNEDGTED TSQPGRVQTF
361TFPETQFIAV TAYQNTDITQ LKIDHNPFAK GFRDNYDTIY TGCDMDRLTP SPNDSPRSQI
421VPGARYAMAG SFLQDQFVSN YAKARFHPGA GAGPGPGTXX RSVPHTNGLL SPQQAEDPGA
481PSPQRWFVTP ANNRLDFAAS AYDTATDFAG NAATLLSYAA AGVKALPLQA AGCTGRPLGY
541YADPSGWGAR SPPQYCGTKS GSVLPCWPNS AAAAARMAGA NPYLGEEAEG LAAERSPLPP
601GAAEDAKPKD LSDSSWIETP SSIKSIDSSD SGIYEQAKRR RISPADTPVS ESSSPLKSEV
661LAQRDCEKNC AKDISGYYGF YSHS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

684 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.