Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TBP / GTF2D Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99989-100
Catalog Number: LS-G99989
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TATA binding protein(TBP) (NM_003194), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GTF2D1 | HDL4 | TATA box binding protein | TATA-binding factor | TATA-box factor | TBP | TFIID | TATA sequence-binding protein | SCA17 | TATA-box-binding protein | GTF2D | TF2D
- NCBI GenBank
- NM_003194
- SwissProt
- P20226
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS ILEEQQRQQQ61QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQAVAAAA VQQSTSQQAT QGTSGQAPQL121FHSQTLTTAP LPGTTPLYPS PMTPMTPITP ATPASESSGI VPQLQNIVST VNLGCKLDLK181TIALRARNAE YNPKRFAAVI MRIREPRTTA LIFSSGKMVC TGAKSEEQSR LAARKYARVV241QKLGFPAKFL DFKIQNMVGS CDVKFPIRLE GLVLTHQQFS SYEPELFPGL IYRMIKPRIV301LLIFVSGKVV LTGAKVRAEI YEAFENIYPI LKGFRKTTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
338 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.