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TBL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G115300-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBL3 (NM_006453), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
UTP13 | WD-repeat protein SAZD | Transducin (beta)-like 3 | Transducin beta-like protein 3 | SAZD | TBL3
Origin Species
Human
NCBI GenBank
NM_006453
SwissProt
Q12788

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
88.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAETAAGVGR FKTNYAVERK IEPFYKGGKA QLDQTGQHLF CVCGTRVNIL EVASGAVLRS
61LEQEDQEDIT AFDLSPDNEV LVTASRALLL AQWAWQEGSV TRLWKAIHTA PVATMAFDPT
121STLLATGGCD GAVRVWDIVR HYGTHHFRGS PGVVHLVAFH PDPTRLLLFS SATDAAIRVW
181SLQDRSCLAV LTAHYSAVTS LAFSADGHTM LSSGRDKICI IWDLQSCQAT RTVPVFESVE
241AAVLLPEEPV SQLGVKSPGL YFLTAGDQGT LRVWEAASGQ CVYTQAQPPG PGQELTHCTL
301AHTAGVVLTA TADHNLLLYE ARSLRLQKQF AGYSEEVLDV RFLGPEDSHV VVASNSPCLK
361VFELQTSACQ ILHGHTDIVL ALDVFRKGWL FASCAKDQSV RIWRMNKAGQ VMCVAQGSGH
421THSVGTVCCS RLKESFLVTG SQDCTVKLWP LPKALLSKNT APDNGPILLQ AQTTQRCHDK
481DINSVAIAPN DKLLATGSQD RTAKLWALPQ CQLLGVFSGH RRGLWCVQFS PMDQVLATAS
541ADGTIKLWAL QDFSCLKTFE GHDASVLKVA FVSRGTQLLS SGSDGLVKLW TIKNNECVRT
601LDAHEDKVWG LHCSRLDDHA LTGASDSRVI LWKDVTEAEQ AEEQARQEEQ VVRQQELDNL
661LHEKRYLRAL GLAISLDRPH TVLTVIQAIR RDPEACEKLE ATMLRLRRDQ KEALLRFCVT
721WNTNSRHCHE AQAVLGVLLR REAPEELLAY EGVRAALEAL LPYTERHFQR LSRTLQAAAF
781LDFLWHNMKL PVPAAAPTPW ETHKGALP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

808 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.