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TBL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TBL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G114409-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBL2 (NM_012453), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Transducin (beta)-like 2 | Transducin beta-like protein 2 | DKFZP43N024 | TBL2 | WBSCR13 | WS-betaTRP
Origin Species
Human
NCBI GenBank
NM_012453
SwissProt
Q9Y4P3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
49.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MELSQMSELM GLSVLLGLLA LMATAAVARG WLRAGEERSG RPACQKANGF PPDKSSGSKK
61QKQYQRIRKE KPQQHNFTHR LLAAALKSHS GNISCMDFSS NGKYLATCAD DRTIRIWSTK
121DFLQREHRSM RANVELDHAT LVRFSPDCRA FIVWLANGDT LRVFKMTKRE DGGYTFTATP
181EDFPKKHKAP VIDIGIANTG KFIMTASSDT TVLIWSLKGQ VLSTINTNQM NNTHAAVSPC
241GRFVASCGFT PDVKVWEVCF GKKGEFQEVV RAFELKGHSA AVHSFAFSND SRRMASVSKD
301GTWKLWDTDV EYKKKQDPYL LKTGRFEEAA GAAPCRLALS PNAQVLALAS GSSIHLYNTR
361RGEKEECFER VHGECIANLS FDITGRFLAS CGDRAVRLFH NTPGHRAMVE EMQGHLKRAS
421NESTRQRLQQ QLTQAQETLK SLGALKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

447 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.