Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TBL1Y / TBL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107463-100
Catalog Number: LS-G107463
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBL1Y (NM_134259), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TBL1 | Transducin beta-like 1 | TBL1Y
- Origin Species
- Human
- SwissProt
- Q9BQ87
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSITSDEVNF LVYRYLQESG FSHSAFTFGI ESHISQSNIN GTLVPPSALI SILQKGLQYV61EAEISINKDG TVFDSRPIES LSLIVAVIPD VVQMRQQAFG EKLTQQQASA AATEASAMAK121AATMTPAAIS QQNPPKNREA TVNGEENGAH EINNHSKPME IDGDVEIPPN KATVLRGHES181EVFICAWNPV SDLLASGSGD STARIWNLNE NSNGGSTQLV LRHCIREGGH DVPSNKDVTS241LDWNSDGTLL AMGSYDGFAR IWTENGNLAS TLGQHKGPIF ALKWNKKGNY VLSAGVDKTT301IIWDAHTGEA KQQFPFHSAP ALDVDWQNNM TFASCSTDMC IHVCRLGCDH PVKTFQGHTN361EVNAIKWDPS GMLLASCSDD MTLKIWSMKQ DACVHDLQAH SKEIYTIKWS PTGPATSNPN421SSIMLASASF DSTVRLWDVE QGVCTHTLMK HQEPVYSVAF SPDGKYLASG SFDKYVHIWN481TQSGSLVHSY QGTGGIFEVC WNARGDKVGA SASDGSVCVL DLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
522 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.