Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TBCEL / E-Like Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G124970-100
Catalog Number: LS-G124970
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBCEL (NM_001130047), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Catastrophin | E-like | EL | LRRC35 | TBCEL
- Origin Species
- Human
- SwissProt
- Q5QJ74
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDQPSGRSFM QVLCEKYSPE NFPYRRGPGM GVHVPATPQG SPMKDRLNLP SVLVLNSCGI61TCAGDEKEIA AFCAHVSELD LSDNKLEDWH EVSKIVSNVP QLEFLNLSSN PLNLSVLERT121CAGSFSGVRK LVLNNSKASW ETVHMILQEL PDLEELFLCL NDYETVSCPS ICCHSLKLLH181ITDNNLQDWT EIRKLGVMFP SLDTLVLANN HLNAIEEPDD SLARLFPNLR SISLHKSGLQ241SWEDIDKLNS FPKLEEVRLL GIPLLQPYTT EERRKLVIAR LPSVSKLNGS VVTDGEREDS301ERFFIRYYVD VPQEEVPFRY HELITKYGKL EPLAEVDLRP QSSAKVEVHF NDQVEEMSIR361LDQTVAELKK QLKTLVQLPT SNMLLYYFDH EAPFGPEEMK YSSRALHSFG IRDGDKIYVE421SKTKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
424 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.